Table 2. Relative IC50 values for inhibition of DynI and DynII by 4a.
| 4a | DynI (brain) | DynI (rec) | DynII (rec) | DynI selectivity ratio |
|---|---|---|---|---|
| Absence of Tween | 0.38 ± 0.05 (5) | 1.1 ± 0.2 (8) | 2.3 ± 0.2 (4) | 2.1 |
| Presence of Tween | 4.9 ± 0.9 (5) | 30.0 ± 8.2 (2) | 92.3 ± 10.9 (5) | 3.1 |
DynI [from brain or recombinant (rec) protein from Sf21 cells] was used at 20 nM and DynII (recombinant protein from Sf21 cells) at 50 nM in the GTPase assay. The selectivity ratio refers to the DynI (rec) IC50 divided by that of DynII (rec). Data are mean ± SEM for the number of independent experiments shown in brackets.