Figure 2.

CLEC‐2‐dependent platelet aggregation is sensitive to cAMP‐mediated inhibition. Washed platelets (2 × 10⁸ per mL) were stimulated with CLEC‐2 mAb or rhodocytin in the presence of increasing concentrations of (A) PGI₂ or (B) GSNO. Representative aggregation traces are shown. Aggregation at 5 min in the presence or absence of either PGI₂ (1 μm for CLEC‐2 mAb, 2 μm for rhodocytin) or GSNO (1 mm) was plotted as mean ± SEM (n = 3–5). Statistical differences were evaluated by two‐way anova and Bonferroni post‐test (**P < 0.01, ***P < 0.001) (C). Washed platelets were stimulated with 0.1 U mL⁻¹ thrombin in the presence of 100 μm GSNO; representative traces are shown (D). Washed platelets were stimulated with HLECs (2 × 10⁶) in the presence or absence of 2 μm PGI₂ or 1 mm GSNO (E) (n = 3).