TABLE 2.
Dataset from a Carbohydrate Microarray Analysis Illustrated in Figure 1
| Probes* | ConA_Mean† | ConA_StDEV | ConA_P Value | TM10_Mean | TM10_StDEV | TM10_P Value | 2G12_Mean | 2G12_StDEV | 2G12_P Value |
|---|---|---|---|---|---|---|---|---|---|
| Markers | 15.62260 | 0.08803 | 14.87944 | 0.03441 | 15.29192 | 0.04377 | |||
| Bg | 10.15045 | 0.35743 | 8.57881 | 0.22869 | 7.47497 | 0.07922 | |||
| P-Man(Y2448) | 10.14444 | 0.10068 | 0.940820863 | 8.89631 | 0.38837 | 0.291392061 | 7.42838 | 0.05436 | 0.271620451 |
| P-Man(Y2448) 1:4 | 10.02619 | 0.11502 | 0.198823146 | 8.89071 | 0.22099 | 0.126203928 | 7.49059 | 0.07411 | 0.75417976 |
| P-Man(Y2448) 1:16 | 10.22374 | 0.22526 | 0.640614989 | 8.79379 | 0.10916 | 0.051393781 | 7.66249 | 0.04541 | 0.008628676 |
| P-Man(Y2448) 1:64 | 10.21577 | 0.23261 | 0.684575226 | 8.73164 | 0.11542 | 0.129350814 | 7.51277 | 0.03622 | 0.2012504 |
| Man9Gn2Asn | 10.56420 | 0.03388 | 3.10365E-09 | 8.84279 | 0.05150 | 0.000186025 | 7.63270 | 0.08081 | 0.069635964 |
| Man9Gn2Asn 1:4 | 10.17306 | 0.02918 | 0.67885688 | 8.57376 | 0.07029 | 0.926499249 | 7.56456 | 0.03088 | 0.013694735 |
| Man9Gn2Asn 1:16 | 10.54928 | 0.02807 | 2.44692E-09 | 8.82917 | 0.20845 | 0.165188048 | 7.56701 | 0.11018 | 0.283223321 |
| Man9Gn2Asn 1:64 | 10.21524 | 0.22238 | 0.674811757 | 8.74372 | 0.12149 | 0.124014225 | 7.51466 | 0.06714 | 0.415769869 |
| Man9-BSA | 15.37738 | 0.04432 | 2.68836E-38 | 11.80805 | 0.34211 | 0.003009395 | 8.64861 | 0.01364 | 6.62876E-23 |
| Man9-BSA 1:4 | 13.77593 | 0.13463 | 1.9426E-06 | 10.44396 | 0.20741 | 0.002335723 | 7.98646 | 0.03816 | 0.000159817 |
| Man9-BSA 1:16 | 12.69489 | 0.15874 | 5.61045E-05 | 10.03899 | 0.08779 | 1.65465E-05 | 7.68872 | 0.02239 | 1.33477E-05 |
| Man9-BSA 1:64 | 12.07279 | 0.13098 | 1.84638E-05 | 9.77254 | 0.09933 | 0.000133201 | 7.64338 | 0.04537 | 0.011461192 |
| Man9-KLH | 15.34345 | 0.26847 | 0.000275556 | 12.69134 | 0.23369 | 0.000585963 | 9.42776 | 0.20184 | 0.003289325 |
| Man9-KLH 1:4 | 14.88474 | 0.09853 | 3.43586E-10 | 11.56674 | 0.10104 | 6.52014E-06 | 8.83664 | 0.08059 | 0.000638523 |
| Man9-KLH 1:16 | 12.79414 | 0.39658 | 0.005414037 | 10.88833 | 0.06345 | 2.40296E-09 | 8.18735 | 0.05987 | 0.00092076 |
| Man9-KLH 1:64 | 12.83746 | 0.37199 | 0.00434454 | 9.93017 | 0.06795 | 3.47497E-07 | 8.25379 | 0.04418 | 0.000136099 |
| M9(2G12)-KLH | 15.61902 | 0.07991 | 1.03991E-14 | 12.33137 | 0.26252 | 0.001048246 | 13.15391 | 0.10919 | 7.64838E-05 |
| M9(2G12)-KLH 1:4 | 14.31340 | 0.02031 | 3.59841E-53 | 10.91850 | 0.16253 | 0.000504444 | 10.46516 | 0.19371 | 0.001263399 |
| M9(2G12)-KLH 1:16 | 14.78672 | 0.01339 | 2.01736E-55 | 10.25366 | 0.09705 | 3.18249E-05 | 10.39027 | 0.11542 | 0.000371526 |
| M9(2G12)-KLH 1:64 | 13.58205 | 0.04742 | 5.36118E-30 | 9.32447 | 0.15719 | 0.007707144 | 9.18056 | 0.20245 | 0.004355371 |
| IM3-BSA | 10.61543 | 0.12902 | 0.005480001 | 9.28675 | 0.02911 | 6.10235E-18 | 7.52614 | 0.01194 | 0.000950827 |
| IM3-BSA 1:4 | 10.71662 | 0.09804 | 0.000213755 | 8.79156 | 0.08706 | 0.023421608 | 7.54167 | 0.01782 | 0.001711139 |
| IM3-BSA 1:16 | 10.54305 | 0.08291 | 0.000367012 | 8.72945 | 0.05673 | 0.012339767 | 7.52081 | 0.02394 | 0.04617645 |
| IM3-BSA 1:64 | 10.64495 | 0.07517 | 1.69228E-05 | 8.72927 | 0.09442 | 0.082587341 | 7.50464 | 0.04693 | 0.392809306 |
| IM6-BSA | 10.43775 | 0.03868 | 1.04823E-05 | 9.23601 | 0.05874 | 1.62475E-06 | 7.46717 | 0.04346 | 0.796089443 |
| IM6-BSA 1:4 | 10.82836 | 0.13053 | 0.00134988 | 8.73535 | 0.04603 | 0.003178209 | 7.48380 | 0.00806 | 0.478895723 |
| IM6-BSA 1:16 | 10.88267 | 0.21738 | 0.015840704 | 8.54127 | 0.13638 | 0.692285058 | 7.43688 | 0.04761 | 0.297995151 |
| IM6-BSA 1:64 | 10.79073 | 0.14876 | 0.004058511 | 8.62936 | 0.10220 | 0.502602184 | 7.49967 | 0.02777 | 0.270985768 |
*
Probe’s initial spotting concentration at ng/mL: P-Man 0.25, Man9Gn2Asn 1.0, Man9-BSA 0.5, Man9-KLH 0.5, M9(2G12)-KLH 0.5, IM3-BSA 0.25, IM6-BSA 0.25.
†
Mean, StDEV and t-test: The log2-transform ed microarray values from triplicate spots for each antigen were included in a statistic analysis. t-Test was performed to examine the differences of significance between each probe and Bg (background), which is background reading of 48 “None” spots in the same microarray image.
IM, isomaltotriose; M9(2G12), [(Man9)4]n; P-Man, phophomannan.