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. Author manuscript; available in PMC: 2014 Aug 20.
Published in final edited form as: J Carcinog Mutagen. 2014 May 31;5:177. doi: 10.4172/2157-2518.1000177

Figure 5.

Figure 5

CDDO-Me induces chromatin modifications. A. Effect on transcriptionally active chromatin markers. Panc-1 and MiaPaCa-2 cells were treated with CDDO-Me (0–0.5 μM) for 5 days and cell lysates were analyzed for acetylated histone H3 (Lys 9), acetylated histone H4, di-methyl H3 (Lys 4) and tri-methyl H3 (Lys 9) by western blotting. B. CDDO-Me induces changes in histone modification in regulatory region of hTERT promoter. Panca-1 cells were treated with CDDO-Me as described above and active chromatin markers interacting with hTERT promoter (acetylated histone H3 (Lys 9), acetylated histone H4, di-methyl H3 (Lys 4) and tri-methyl H3 (Lys 9) were analyzed by ChIP-PCR assay as described in Materials and Methods. No antibody controls were included to verify ChIP efficacy. The experiment was repeated two times.