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. 2014 Aug 26;3:e03245. doi: 10.7554/eLife.03245

Figure 3. Increased energy expenditure and oxygen consumption in global and fat-specific Alk7 knock-out mice on a high fat diet.

(A and B) Energy expenditure assessed by calorimetric measurements in global Alk7−/− (A) and fat-specific Alk7fx/−::Ap2CRE (B) knock-out mice after 16 weeks on a high fat diet (HFD). N = 8 mice per group in (A), N = 6 in (B). (C and D) Oxygen consumption assessed by calorimetric measurements in global Alk7−/− (C) and fat-specific Alk7fx/−::Ap2CRE (D) knock-out mice after 16 weeks on HFD. N = 8 mice per group in (C), N = 6 in (D). (E) Daily food intake in fat-specific Alk7fx/−::Ap2CRE knock-out mice during 16 weeks on HFD. N = 6 mice per group. (F and G) Physical activity assessed as ambulation in global Alk7−/− (F) and fat-specific Alk7fx/−::Ap2CRE (G) knock-out mice after 16 weeks on HFD. N = 8 mice per group in (F), N = 6 in (G). *p < 0.05; **p < 0.01; NS, non-significant (mutant vs control). All error bars show mean ± SEM.

DOI: http://dx.doi.org/10.7554/eLife.03245.008

Figure 3.

Figure 3—figure supplement 1. Alk7 deletion does not result in enhanced 'browning' of subcutanous adipose tissue.

Figure 3—figure supplement 1.

(A and B) Relative mRNA expression of brown adipose tissue (BAT) markers Ucp1 and Elovl3 in subcutaneous adipose tissue of Alk7fx/fx::Ap2CRE fat-specific knock-out mice (A), global Alk7 knock-out mice (B), and corresponding controls. N = 4 mice per group in (A), N = 5 in (B). (C and D) Relative mRNA expression of brown adipose tissue (BAT) markers Ucp1 (C) and Elovl3 (D) in subcutaneous adipose tissue of wild type and global Alk7 knock-out mice following injection of the β3-AR-specific agonist CL316243 or vehicle as indicated. N = 4 mice per group. *p < 0.05; **p < 0.01; ***p < 0.001; NS, non-significant (mutant vs control). All error bars show mean ± SEM.
DOI: http://dx.doi.org/10.7554/eLife.03245.009