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A putative binding site of NF-Î B of cyclin B1 first intron. A putative NF-Î B binding site was located in the first intron of human cyclin B1 gene (GenBank #M25753). The marked regions (CB1F2 and CB1R2) were designed to produce fragment of cDNA from human breast cancer MCF-7 cells used for construction of cyclin B1-first-intron luciferase reporters (pGL3CB1EI1).
