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. Author manuscript; available in PMC: 2014 Aug 20.
Published in final edited form as: Anticancer Res. 2004 Sep-Oct;24(0):2657–2663.

Figure 5.

Figure 5

Over expression of human SOD2 genes inhibited luciferase activity of pCGLCB1EI1 and NF-Î B. MnSOD proteins were dose-dependently increased with SOD2 gene transfection (A). Gene expression vectors containing human SOD2 (11) gene were transiently transfected into MCF-7 cells and MnSOD protein levels were estimated using Western blotting. Luciferase reporters controlled by NF-Î B (B) or pGL3CB1EI1 (C) were co-transfected with (1-4 mg/flask) human MnSOD gene expression vector (SOD2) and luciferase activities were measured 24 h after exposure to 5 Gy IR. Luciferase activity was normalized to protein concentration (mean±S. D., n=4; **p<0.01).