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. Author manuscript; available in PMC: 2014 Oct 15.

Published in final edited form as: J Neuroimmunol. 2013 Aug 1;263(0):20–27. doi: 10.1016/j.jneuroim.2013.07.009

Concentrations of pro-inflammatory cytokines (A) IL-6 and (B) IFN-γ from the cell culture supernatant. Splenocytes were isolated from the spleens of MOG-induced EAE mice on day 30. The three different groups consisted of PBS-, MOG-BPI-, or MVB_MOG/PLP- treated mice on days 4, 7, and 10. The pooled splenocytes (n = 3 mice) were stimulated in vitro with PLP_139-151, and supernatant was isolated 72 hours later for cytokine detection (*p < 0.01).

Concentrations of pro-inflammatory cytokines (A) IL-6 and (B) IFN-γ from the cell culture supernatant. Splenocytes were isolated from the spleens of MOG-induced EAE mice on day 30. The three different groups consisted of PBS-, MOG-BPI-, or MVB_MOG/PLP- treated mice on days 4, 7, and 10. The pooled splenocytes (n = 3 mice) were stimulated in vitro with PLP_139-151, and supernatant was isolated 72 hours later for cytokine detection (*p < 0.01).