Figure 5.
Incorporation of [3H] thymidine in splenocytes isolated from PLP-induced EAE mice on day 30. EAE-free mice were stimulated with ConA, medium, PLP (2 μM), or MOG (2 μM) and the remaining treated groups were stimulated with only medium, PLP (2 μM), and MOG (2 μM). The three treatment groups consisted of PBS-, PLP-BPI-, or MVBMOG/PLP- treated mice on days 4, 7, and 10. Each group (n = 3) was pulsed with [3H] thymidine 72 hours after stimulation and incorporated radioactivity was measured the following day.