. 2014 Jul 23;53(32):5261–5271. doi: 10.1021/bi500704p

Table 1. Quantitation of Heme Bound to Purified HCCS^a.

		total heme
putative domain	HCCS variant	expressed alone	coexpressed with cytochrome c acceptor
	WT	1	1
Domain I	W118A	1.2 ± 0.06	0.7 ± 0.02
	Y120A/P121A	<0.10	<0.10
	Y120A	<0.10	1.0 ± 0.06
	P121A	0.5 ± 0.14	0.7 ± 0.13
	N128A/M130A	0.9 ± 0.03	0.6 ± 0.07
	N128A	1.3 ± 0.51	0.8 ± 0.17
	M130A	1.1 ± 0.18	1.2 ± 0.07
Domain II	N155A	1.0 ± 0.09	0.5 ± 0.12
	E159K	0.5 ± 0.10	0.3 ± 0.08
	E159A	0.4 ± 0.07	0.3 ± 0.05
	E159D	1.2 ± 0.23	0.9 ± 0.09

Bound heme was determined from reduced pyridine hemochrome spectra using 100 μg of total purified protein. Each value is relative to the amount of total heme purified with WT HCCS under each respective condition, which has been set to 1. Data shown represent the average of at least two separate experiments ± SEM.

Table 1. Quantitation of Heme Bound to Purified HCCSa.

Table 1. Quantitation of Heme Bound to Purified HCCS^a.