Figure 5.

Qdot assay analytics comparing native (black), phosphorylated (blue), and OM treated (red) βMys. The average force, ⟨F⟩, in units of force [uf and eq 5 (A)], dynamic velocity, u [eq 6 (B)], and average power, ⟨P⟩ [eq 7 (C)], were computed as a function of the relative step-frequency for the long step, ω_L, assuming relative step-frequency for the short step, ω_S, was constant and taken from Table 1. System optimization calls for maximizing ⟨P⟩ with βMys activation. Panel C shows that maximal ⟨P⟩ occurs for maximal ω_L. Activated ⟨P⟩ due to phosphorylation (blue arrow) is larger than activated ⟨P⟩ by OM treatment (red arrow). Blue and red arrows follow the changes in ⟨F⟩ and u due to phosphorylation and OM treatment, respectively. Arrows begin and end on values listed in Table 1.