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. 2014 Jul 29;86(16):8149–8157. doi: 10.1021/ac501380s

Figure 2.

Figure 2

Efficient and reproducible extraction and sample preparation for the 60 animals. (A) Protein extraction yields from liver samples using a unique Polytron/sonication protocol with a cocktail of high-concentration of detergents. (B) The peptide recovery of the precipitation/on-pellet-digestion procedure. Peptide recovery was determined by a modified BCA method described previously.32