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CEST images of phantoms on an MRI 4.7 T scanner with a pulse train composed of five Gauss pulses at 12 μT for 1 s each, interpulse delay of 200 μs applied symmetrically about the bulk water resonance (±59 ppm). Sample A consisted of 20 mM HEPES pH 7.4 and 100 mM NaCl. All other solutions contained 4 mM [Co(L)]2+ in (B) 20 mM HEPES pH 7.4 and 100 mM NaCl, (C) rabbit serum, (D) 20 mM HEPES pH 7.4 and 100 mM NaCl in 4% agarose gel (w/w) pH 7.3–7.4 at 37 °C.
