Figure 3.

Analytical sensitivity studies. Limit of detection for (A) H1N1 Influenza A/Brisbane/59/2007, (B) H1N1 Influenza A/Solomon Islands/3/2006 (C) H3N2 Influenza A/Aichi/2/1968. Fluorescence intensity for A/Brisbane/59/2007 was measured as previously described for Figure 2. Fluorescence intensity was measured by the Genepix scanner using ferret hyperimmune sera to influenza A/Solomon Islands/3/2006 (H1N1) and antiferret rhodamine labeled antibody; polyclonal antiserum chicken to A/Aichi/2/1968 (H3N2) and Alexa Fluor 633 labeled antichicken. A/Solomon Islands/3/2006 was scanned at 532 nm and A/Aichi/2/1968 at 635 nm. All experiments were performed in triplicate.