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. 2014 Aug 4;86(16):8441–8447. doi: 10.1021/ac5020657

Figure 4.

Figure 4

Sensitivity and specificity of the LAMP reaction. The sensitivity was evaluated on (A) HNB visualization of color change and (B) 2% agarose gel electrophoresis analysis of the LAMP products. Specificity was assessed by detecting 2 × 106 pfu mL–1 helper phage M13K07 and 1 mg μL–1 M13KE vector, the result was observed by (C) color change and (D) 2% agarose gel electrophoresis. M represented 2000 bp DNA mark; 1 represented positive control; 8 and CK represented negative control; 2 to 7 respectively represented 8.5 × 104, 1.7 × 104, 8.5 × 103, 1.7 × 103, 8.5 × 102, and 1.7 × 102 pfu mL–1 C11-2.