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Self-interactions of SRCR domains at various concentrations (0.250–2 μm) with immobilized iSRCR1 (A and C) and iSRCR123 (B and D) on CM5 sensor chip were analyzed using BIA-Evaluation software. The analytes iSRCR1 and iSRCR123 demonstrated nanomolar affinity interaction with SRCRs, clearly indicating the self-adhesion property among SRCRs.
