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. 2014 Jun 25;289(32):21888–21895. doi: 10.1074/jbc.M114.582866

FIGURE 3.

FIGURE 3.

Identification of putative miR-23b/27b/24 cluster targets. A, average gene expression (expressed as fold change) for candidate target genes from microarray analysis of 4TO7-miR-23b/27b/24 cells relative to vector control. B, qRT-PCR mRNA expression levels for putative target genes in 4TO7 vector cells or miR-23b/27b/24 overexpressing cells. **, p < 0.05; **, p < 0.01 by Student's t test. Data represent average ± S.D. C, normalized firefly luciferase expression from reporter plasmids containing indicated 3′-UTR 24 h after co-transfection with control or miR-23b/27b/24 miRNA oligonucleotides. **, p < 0.01. Data represent average ± S.D. D, schematic representing the predicted miR-27ab and miR-24 binding sites within the human PSAP 3′-UTR. MiR-24 contains two predicted sites, referred to here as 1 and 2, whereas miR-27ab contains one predicted binding site. E, normalized firefly luciferase (Luc) expression from PSAP reporter constructs similar to following site-directed mutagenesis of predicted miRNA binding sites and co-transfection with miR-27b or miR-24 as indicated. *, p < 0.05; **, p < 0.01 by Student's t test. Data represent average ± S.D.