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. 2014 Jun 10;289(32):21926–21936. doi: 10.1074/jbc.M113.532358

FIGURE 1.

FIGURE 1.

Ninjurin1 deficiency decreases motility of bone marrow-derived macrophages. A, BMDMs isolated from wild-type (WT) mice were cultured and then immunostained using an antibody against F4/80 (green), a macrophage marker, or an IgG control. The nuclei of BMDMs were counterstained using Hoechst 33342 (blue). Scale bar, 50 μm. B, WT and Ninjurin1-deficient BMDMs were subjected to Western blot analysis performed using anti-Ninjurin1 antibody (Ab1–15). C and D, basal motility of WT and Ninjurin1-deficient BMDMs. Images of WT and Ninjurin1-deficient BMDMs were obtained every 10 min for 6 h on the uncoated surface of a six-well plate. Trajectory plot (C) and dot charts (D) show the velocity and Euclidean distance of 40 cells. **, p < 0.01.