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. 2014 Jun 10;289(32):21926–21936. doi: 10.1074/jbc.M113.532358

FIGURE 7.

FIGURE 7.

Rac1 activation is required for Ninjurin1-mediated formation of filopodial protrusions. A, analysis of the activity of small GTPases RhoA, Rac, and Cdc42 in siNinj1 by using an enzyme-linked assay. *, p < 0.05. B and C, filopodial protrusions expressing RFP-Fascin1 (arrowheads) in GFP- or GFP-mNinj1 Raw264.7 cells after treatment with the Rac1 inhibitor NSC23766 (20 μm, 3 h) (B). Representative images are shown. Arrowheads indicate filopodial structures expressing RFP-Fascin1 (C). The statistical analysis was performed using data from ∼40 cells. Scale bar, 10 μm. N.S., no significance; ***, p < 0.001.