TABLE 1.
Steady state kinetic parameters for the oxidation of urate and thiocyanate by LPO
Km and Vmax were determined by fitting the kinetic data in Fig. 5 according to Michaelis-Menten kinetics. The catalytic rate constant kcat was calculated by dividing Vmax by the concentration of LPO heme. The specificity constant kx− was calculated by dividing kcat by Km.
| LPO + urate (initial phase) | LPO + urate (secondary phase) | LPO + SCN− | |
|---|---|---|---|
| Km (μm) | 100 ± 20 | 220 ± 30 | 340 ± 60 |
| kcat (s−1) | 0.56 ± 0.06 | 0.13 ± 0.01 | 180 ± 20 |
| kx− (m−1 s−1) | 5.6 × 103 | 580 | 5.5 × 105 |