. 2014 Jun 19;289(32):21995–22007. doi: 10.1074/jbc.M114.548792

TABLE 1.

S. pombe strains used in this work

All strains were generated in this study except as indicated in the footnotes.

Strains	Genotypes
YS64	h⁻ leu1-32 ade6M210 ura4D-18 his3D1
SM116	h⁻ gef3::ura4⁺ leu1-32 ade6M210 ura4D-18 his3D1
NG69	h⁻ cdc25-22 leu1-32 ura4D18
SM176	h⁻ gef3::ura4⁺ cdc25-22 leu1-32 ura4D18
PG153^a	h⁺ gef1::kanMX6 leu1-32 ura4D18
SM143	h⁻ gef3::ura4⁺ gef1::kanMX6 leu1-32 ura4D18
CL5	h⁻ gef2::ura4⁺ leu1-32 ade6M210 ura4D-18 his3D1
SM172	h⁻ gef2::ura4⁺ gef3::his3⁺ leu1-32 ade6M210 ura4D-18 his3D1
PG154^a	h⁺ scd1::kanMX6 leu1-32 ura4D18
SM157	h⁻ gef3::ura4⁺ scd1::kanMX6 leu1-32 ura4D18
VT14	h⁻ rgf1::his3⁺ leu1-32 ade6M210 ura4D18 his3d1
SM148	h⁻ gef3::ura4⁺ rgf1::his3⁺ leu1-32 ade6M210 ura4D1 his3d1
PG1	h⁻ rgf2::ura4⁺ his3d1 ura4D18 leu1-32 ade6M210
SM150	h⁻ gef3::ura4⁺ rgf2::ura4⁺ his3d1 ura4D18 leu1-32 ade6M210
MS264	h⁻ ehs2–1 leu1-32 ura4D18
SM152	h⁻ gef3::ura4⁺ ehs2–1 leu1-32 ura4D18
SM217	h⁻ ura4⁺:GFP-gef3⁺ leu1-32 ura4D18
SM267	h⁻ GFP-gef3:KanMX6 rlc-tdTomato:natMX6 ura4D18
SM263	h⁻ ura4⁺:tdTomato-gef3⁺ leu1-32 ura4D18
PPG2772^a	h⁻ spn3-GFP:kanMX6 leu1-32 ade6M210 ura4D-18 his3D1
SM197^b	h⁻ mid2-GFP:kanMX6 leu1-32 ura4D-18
SM297	h⁻ spn3-GFP:kanMX6-tdTomato gef3:ura4⁺ leu1-32 ura4D-18
SM295	h⁻ mid2-GFP:kanMX6 tdTomato-gef3:ura4⁺ leu1-32 ura4D-18
SM277^c	h⁻ GFP-gef3:KanMX6 spn1–5::ura4⁺ura4D18
SM253	h⁻ GFP-gef3:KanMX6 mid2::ura4⁺ ura4D18 leu1-32
SM273	h⁻ spn3-GFP:kanMX6 gef3::ura4⁺ leu1-32 ade6M210 ura4D-18 his3D1
SM229	h⁻ mid2-GFP:kanMX6 gef3::ura4⁺ leu1-32 ura4D-18
SM271	h⁻ GFP-gef3:ura4⁺ exo70-RFP:kanMX6 leu1-32 ura4D18
SM259	h⁻ GFP-gef3:KanMX6 exo70::ura4⁺ ura4D18 leu1-32
SM261	h⁻ GFP-gef3:KanMX6 sec8–1 ura4D18 leu1-32
MBY797^b	h⁻ sec6-GFP:ura4⁺ leu1-32 ura4D18
MBY856^b	h⁻ sec8-GFP:ura4⁺ leu1-32 ura4D18
SM244	h⁻ sec6-GFP:ura4⁺ gef3::KanMX6 leu1-32 ura4D18
SM245	h⁻ sec8-GFP:ura4⁺ gef3::KanMX6 leu1-32 ura4D18
SM242^a	h⁻ kanMX6-GFP-rho3⁺ leu1-32 ura4D18
SM594	h⁻ kanMX6-GFP-rho3⁺ gef3::ura4⁺ leu1-32 ura4D18
PPG3740^a	h⁺ rho3::ura4⁺ leu1-32 ade6M210 ura4D18
EM257	h⁻ rho3::ura4⁺ gef3::KanMX6 leu1-32 ade6M210 ura4D18
SM686	h⁻ kanMX6-GFP-rho3⁺ sid4-RFP:natMX6 leu1-32 ura4D18
EM83^a	h⁹⁰ leu1:GFP-Psy1
EM256	h⁻ GFP-Psy: leu gef3::KanMX6
MBY919^b	h⁻ exo70::ura4⁺ leu1-32 ura4D-18
SM243	h⁻ exo70::ura4⁺ gef3::KanMX6 leu1-32 ura4D-18
MBY887^b	h⁺ sec8–1 leu1-32 ura4D18
SM226	h⁻ sec8–1 gef3::ura4⁺ leu1-32 ura4D18
PPG2567^a	h⁻ for3::KanMX1 leu1-32 ura4D18
SM300	h⁻ for3::KanMX1 gef3::ura4⁺leu1-32 ura4D18
SM708^d	h⁻ apm1::ura4⁺ leu1-32 ura4D18
SM721	h⁻ gef3::KanMX6, apm1::ura4⁺ leu1-32

^a From P. Pérez, IBFG, University of Salamanca.

^b From C. R. Vázquez de Aldana, IBFG, University of Salamanca.

^c From J. Bähler, Wellcome Trust, UK.

^d From H. Valdivieso, IBFG, University of Salamanca.