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Expression of vectors containing a unique uracil in HeLa-derived SMUG1 (SMUG1sh-c22) and TDG (TDGsh-c6) knockdown cell lines. TS and NTS refer to the DNA strand containing uracil. A and B, representative flow cytometry data (12 h post-transfection) for the U:A (A) and U:G (B) constructs. No sh, empty vector. C, relative EGFP expression (U:A/T:A and U:G/C:G) for two independent preparations of each expression construct at the indicated times post-transfection. Data are mean and range.
