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Bacterial expression plasmid constructs used for studies of protein-protein interactions
| Name | Coding sequence (cDNA) | Backbone |
|---|---|---|
| pE1 | cs-p58-His6, cs-SUMOp180C | pETDuet-1 |
| pE2 | cs-p58-His6 | pETDuet-1 |
| pE3 | cs-p49, cs-p58 | pETDuet-1 |
| pE4 | cs-p49, cs-p58N (encoding amino acids 1–265) | pETDuet-1 |
| pE5 | cs-p49, cs-p58C (encoding amino acids 266–509) | pETDuet-1 |
| pC1 | cs-p70-His6, cs-SUMOp180C, cs-p66N | pColaDuet-1 |
| pC2 | cs-p70-His6 | pColaDuet-1 |
| pC3 | cs-SUMOp180C | pColaDuet-1 |
