TABLE 2.
Plasmids used in this study
| Plasmid | Description | Ref. or source |
|---|---|---|
| pFL38 | CEN-ARS (URA3) | 46 |
| pJOD10 | p416 GAL1-GAP1-GFP (URA3) | 19 |
| pCJ038 | p416 GAL1-GAP1K9R,K16R-GFP (URA3) | Lab collection |
| pCJ004 | p416 GAL1-GAP1 (URA3) | Lab collection |
| pCJ034 | p416 GAL1-GAP1K9R,K16R (URA3) | Lab collection |
| pEL003 | YCp GAP1-GFP (URA3) | 45 |
| pEL005 | YCp GAP1K9R,K16R-GFP (URA3) | 45 |
| pEL018 | YCp GAP1K9R-GFP (URA3) | 10 |
| pEL021 | YCp GAP1K16R-GFP (URA3) | 10 |
| pRS426-GST | pRS426-pADH-GST (URA3) | 47 |
| pRS426-GST-BMH2 | pRS426-pADH-GST-BMH2 (URA3) | 47 |
| pMA74 (Gap1-112) | p416 GAL1-GAP1ITIP33AAAA-GFP (URA3) | 24 |
| pMA47 (Gap1-106) | p416 GAL1-GAP1EKNN11AAAA-GFP (URA3) | 24 |
| pMA51 (Gap1-108) | p416 GAL1-GAP1KHNG19AAAA-GFP (URA3) | 24 |
| pMA03 (Gap1-103) | p416 GAL1-GAP1T567A-GFP (URA3) | 24 |
| pMA28 (Gap1-132) | p416 GAL1-GAP1EIAE583AAAA-GFP (URA3) | 24 |
| pMA31 (Gap1-134) | p416 GAL1-GAP1VDLD575AAAA-GFP (URA3) | 24 |
| pMA33 (Gap1-135) | p416 GAL1-GAP1GRRE571AAAA-GFP (URA3) | 24 |
| pMA35 (Gap1-136) | p416 GAL1-GAP1AEKM563AAAA-GFP (URA3) | 24 |
| pNG29 (Gap1-157) | p416 GAL1-GAP1AEQR408AAAA-GFP (URA3) | 24 |
| pNG38 (Gap1-162) | p416 GAL1-GAP1ALAA480AAAA-GFP (URA3) | 24 |
| pNG41 (Gap1-164) | p416 GAL1-GAP1LDEL488AAAA-GFP (URA3) | 24 |
| pNG42 (Gap1-165) | p416 GAL1-GAP1SFK491AAA-GFP (URA3) | 24 |
| pNG53 (Gap1-170) | p416 GAL1-GAP1LVG426AAA-GFP (URA3) | 24 |
| pMYC016 (Gap1-134–135) | p416 GAL1-GAP1GRREVDLD571AAAAAAAA-GFP (URA3) | This study |
| pMA185 | p416 GAL1-CAN1-GFP (URA3) | Lab collection |
| pNAM001 | YCp LYP1-GFP (URA3) | 48 |
| pFL38-gF-GFP | pFL38 GAL1-FUR4-GFP (URA3) | Lab collection |