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. 2014 Jun 24;289(32):22117–22127. doi: 10.1074/jbc.M114.566893

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — The GPR30-MAGUK-AKAP5 complex is colocalized with the PKA RIIβ regulatory subunit in the plasma membrane. HEK cells transfected with PKA RIIβ-GFP (RIIβ-GFP) together with GPR30 or GPR30ΔSSAV were subjected to live staining with M1 FLAG antibodies. The results are representative of experiments performed at least three times. The individual and merged images (Merge) were collected using a Nikon Eclipse confocal microscope, ×60 objective, and 50-μm zoom. The arrows indicate cells coexpressing PKA RIIβ-GFP either with GPR30 or GPR30ΔSSAV.