FIGURE 7.

Intracellular methamphetamine prevents the dopamine-induced inward current. A, schematic depicts the experimental configuration. The dopamine-induced inward current was measured in CHO-YFP-DAT cells when internal solution or internal solution containing amphetamine (10 μm; n = 4), or methamphetamine (10 μm; n = 5) was dialyzed into the cell. Dopamine (10 μm) was bath-applied. The experiments were performed as described in Fig. 6. B and C, representative traces at −100mV. D, the bar graph compares the dopamine-induced inward current at −100 mV and −80 mV after intracellular delivery of methamphetamine (10 μm; n = 5) or amphetamine (10 μm; n = 4). Intracellular methamphetamine, but not amphetamine, decreases the dopamine-induced, DAT-mediated inward current. *, p < 0.05, one-way ANOVA followed by Tukey's post hoc test.