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. 2014 Jun 20;289(32):22258–22267. doi: 10.1074/jbc.M114.588046

FIGURE 5.

FIGURE 5.

Colocalization test with CtxB, role of DYN2 and phagocytosis in exosome uptake. A, merging of bright-field images and confocal images of PC12 cells incubated with exosomes and FITC-CtxB (green) for 10 min, 30 min, or 1 h. Yellow indicates the colocalization of exosomes and CtxB (scale bars, 15 μm). B, quantification of the colocalization of exosomes and CtxB at various time points. C, Western blot results of DYN2 48 h after transfection of NC or DYN2-shRNA. D, DYN2 expression level was quantified by determining the gray value. E, confocal images of cells transfected with NC or DYN2-shRNA for 48 h, and then incubated with exosomes at 37 °C for 3 h. Scale bar, 20 μm. F, exosome uptake was quantified by determining the fluorescence intensity. G, merging of bright-field images and confocal images of PC12 cells incubated with exosomes and FITC-labeled latex beads (green) at 37 °C for 3 h. Scale bar, 50 μm. In all the images, red refers to DiD-labeled exosomes, and blue indicates nuclei. For all the quantification plots, mean ± S.D. of three independent experiments is shown.