TABLE 1.

Primers used for sequencing the cDNAs of chimeric viral RNAs

The cDNAs were amplified from chimeric viral RNAs with or without acquired mutations by RT-PCR as described under “Experimental Procedures.”

Primer numbera	Primer sequence
9F	TCT ACG TGG ACC GAC AAA GAC
222R	TCC TCG TCC CTG CAG CAT TCC
962F	AGA GAC TTT GTA GAA GGG GTT
1100R	AGT GGC AGG AGG TTG TTT GGC TTC
1767F	ACT GTT CAC AGG ACA TCT CAA
2300R	GAC TCC TAT GAG GAT TTT CAT
2970F	CAT GTC AGC GGC CAT AAA AGA
3260R	CAC CAC TGT GGT TCC TTC GCA
4000F	TGT CCG TTT CCC CAC TGT TCT
4200R	CAT TCT TTA GGA GTG AAC TGG
5000F	CAT ATG TGA GTG CTA TAG CCC
5200R	CGA CTC TAG TGG GGG CCA GGA
5700R	GGT TCT AGT CTT GAC ATA CTC
6000F	CTC CTA GAT AAC ATC AAC ACA
6200R	CAA AAC ACC ACC TTC TGT CTG
7000F	CTC AGT GAA CGT GTC CCT AAC
7250F	AAA CCC AAC TGT CGA TG
8200R(D4)b	GTT CCT GGA CAG CGG GCA TCT
8500F	AAC AAA CTG GAT CAG CAT C
8600R	TGT CAT TGC CAT CTG TGT CAC
9556F	CAA GAA TGG CCA TCA GTG GAG
9760R	GGT TTC TGC ATG GAA CTA CAA
10455F	TCT GGG AGG CCA CAA ACC ATG
10620F	CAG CAT ATT GAC GCT GGG AAA
3′endR	AGA ACC TGT TGA TTC AAC AGC

^a F and R refer to forward and reverse primers. The primer numbers refer to their positions in the D2 genome of New Guinea C-strain (28).

^b D4 primer is in the MT domain of NS5.