TABLE 5.

In vitro MT activities of recombinant WT D2 NS5 and NS5 D4MT/D2POL chimeric proteins

The recombinant proteins are expressed in E. coli and purified as described under “Experimental Procedures.”

Species	D2 NS5FLa	D2 NS5POL	D4MT/D2POL	D4MT K74I/D2POL
	%	%	%	%
7MeGpppA2′-OMe	44.98 ± 0.79	0	44.76 ± 2.90	43.72 ± 6.45
GpppA2′-OMe	43.22 ± 6.89	0	47.31 ± 2.03	46.04 ± 6.73
GpppA	11.80 ± 6.82	100 ± 0	7.92 ± 4.58	10.23 ± 5.91

^a Values represent mean and S.E. of four values from two independent experiments (Fig. 8). The total radioactivity in each lane was normalized to 100%, and the percentage distribution of the radioactivity in various spots was calculated.