FIGURE 3.

Altered expression and production of IL-6 in response to TNFα in human Niemann-Pick fibroblasts. A, equal numbers of control (Con) or Niemann-Pick type A disease fibroblasts were plated and treated the next day with TNFα for 18 h. Cultured media were assessed for IL-6 by ELISA (n = 2). B and C, WT and NPD fibroblasts were treated with TNF (20 ng/ml) or untreated for 90 min. RNA and cDNA were prepared, and IL-6 mRNA (B) and hnRNA (C) were measured as described under “Experimental Procedures” (n = 2). Error bars, S.E.