TABLE 1.
Primer sequences used in cloning
Underlined sequences represent a site for restriction enzyme.
| Plasmid | Vector | Restriction enzyme | Primer (5′-3′) |
|---|---|---|---|
| 4HA | pBabe-puro | BglII | GGAAGATCTATGGCTTACCCATACGATG |
| SnaBI | GGCCTACGTAAGCGTAATCTGGAACGTC | ||
| myc-MTF-1 | pBabe-puro | SnaBI | GGCTACGTAATGGAGCAGAAACTCATCTCTGAAGAGGATCTGGGGGAACACAGT |
| SalI | GGCGGCGTCGACTCACTTGGAGAAGCT | ||
| HA-MTF-1 | pBabe-4HA | SnaBI | GGCGGCTACGTAATGGGGGAACACAGT |
| SalI | GGCGGCGTCGACTCACTTGGAGAAGCT | ||
| HA-PR110 | pBabe-4HA | SnaBI | GGCGGCTACGTAATGGACGAGCAGGCG |
| SalI | GGCGGCGTCGACTCATACAAAGACTTT | ||
| HA-B56β | pBabe-4HA | SnaBI | GGCGGCTACGTAATGGAGACGAAGCTG |
| SalI | GGCGGCGTCGACCTAGCTCTGACC | ||
| MT1A | pGL3 | XhoI | GGCGGCCTCGAGGGTGGCTGTGAATGA |
| HindIII | GGCGGCAAGCTTCCAAGCAAGAAGTTG | ||
| HA-MTF-1-S5A | pBabe-4HA | SnaBI | ATGGGGGAACACGCTCCAGACAAC |
| SalI | GTTGTCTGGAGCGTGTTCCCCCAT | ||
| HA-MTF-1-T252A | pBabe-4HA | SnaBI | AAGCACATTCGAGCTCATACAGGGGAA |
| SalI | TTCCCCTGTATGAGCTCGAATGTGCTT | ||
| HA-MTF-1-T254A | pBabe-4HA | SnaBI | ATTCGAACTCATGCAGGGGAAAAGCCA |
| SalI | TGGCTTTTCCCCTGCATGAGTTCGAAT | ||
| HA-MTF-1-S305A | pBabe-4HA | SnaBI | AGCACTCAATACAGTCTCAAAAGTCAC |
| SalI | GTGACTTTTGAGACTGTATTGAGTGCT | ||
| HA-MTF-1-S620A | pBabe-4HA | SnaBI | CAGATTGGCCTCGCTGTTCCTGTGATC |
| SalI | GATCACAGGAACAGCGAGGCCAATCTG |