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Effect of ZO protein depletion on the expression of the YAP target gene CTGF in MDCK cells. Relative levels of mRNA expression of CTGF in sparse (red) and confluent (yellow) MDCK WT cells, ZO-1KD clones (ZO-1KD1, ZO-1KD2, ZO-1KD3), ZO-2KD cells, ZO-3KD, and ZO-3 rescue clone, and double KD clones (dKD-1/2/3), as determined by qRT-PCR analysis. Note that CTGF activity in sparse cells is lowered following ZO-2KD, but not following double KD of ZO-1 and ZO-2.
