FIGURE 2.

Kinetic traces of COH formation during oxidation of CBA by TyrOOH. Inset, dependence of pseudo-first order rate constants of COH formation on the concentration of CBA. Reaction mixtures containing tyrosine (1 mm), hypoxanthine (1 mm), catalase (0.25 kilounits/ml), HRP (0.5 units/ml), and XO (0.6 milliunits/ml) in phosphate buffer (50 mm, pH 7.4) and DTPA (0.1 mm) were incubated for 1 h. After incubation, SOD (0.1 mg/ml) and CBA (0.05–0.8 mm) were added, and fluorescence measurements were started.