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. 2014 Aug 20;9(8):e104699. doi: 10.1371/journal.pone.0104699

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Triple-labeled confocal microscopic image analyses were used to study the distribution and relative levels of albumin (green), AGE (red), and DAPI (blue) in the hippocampus Cornu Ammonis Area1 (CA1), Cornu Ammonis Area2 (CA2), Cornu Ammonis Area3 (CA3), Dendate Gyrus (DG) of the control rats (A) and binge-alcohol exposed rats (B). Scale bar = 50 µm. (C, D) Co-immuonoprecipitation analysis of AGE-ALB in the hippocampus (C) and cerebellum (D) in control or alcohol-exposed rats on days 9 and 11. Scale bar = 50 µm.