Skip to main content
. 2014 Aug 20;9(8):e104763. doi: 10.1371/journal.pone.0104763

Figure 1. Genz-529648 reduces P. aeruginosa stimulated IL-8 mRNA expression and protein release.

Figure 1

Panels A and B. Genz-529648 reduces P. aeruginosa stimulated IL-8 mRNA expression. IB3-1 (A) and CuFi-1 (B) cells were treated with a range of doses of Genz-529648 (1–100 nM) or solvent alone for 1 hour and then infected with PAO1 for 4 hours at 37°C. The inflammatory response was evaluated by studying the expression of IL-8 mRNA, which was measured by Real-time qPCR and obtained by comparing the ratio IL-8 and the housekeeping gene GAPDH between non-infected and infected cells. The results are expressed as the % of untreated cells and represent the mean ± standard error of the mean of 4 independent experiments in duplicate. Comparisons between groups were made by using Student’s t tests. Panels C and D. Genz-529648 reduces the P. aeruginosa induced IL-8 secretion. IB3-1 (C) and CuFi-1(D) cells were treated with Genz-529648 (100 nM) for 1 hour prior to infection with heat killed PAO1 for 24 hours. Data reported are the mean ± standard error of the mean of 4 independent experiments in duplicate. Comparisons between groups were made by using Student’s t tests.