Figure 8. Infection with PAO1 increases whole cell ceramides in CF bronchial epithelial cells.

IB3-1 (A) and CuFi-1 (B) cells were treated with [2 µM] miglustat, [10 nM] Genz-529648 or solvent alone and infected with PAO1 as indicated in figure 3. After infection, whole cell ceramides were analyzed by LC-MS and LC-MS/MS methods as described in the online supplement. The data reported are the mean ± SE of three independent experiments performed with both cell lines. Comparisons between groups were made by using Student’s t tests.