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. 2014 Aug 20;9(8):e105520. doi: 10.1371/journal.pone.0105520

Figure 2. Screening of vectors in Balb/c mice.

Figure 2

A) Balb/c mice were immunised i.m. with 50 µg of DNA plasmid pIMM.TIP or pIMM.TIP_m4-1BBL, spleens harvested at day 7 and the response to Pb9 and P15 measured by ELISpot. B) Balb/c mice were immunised i.d. with 106 PFU of MVA.TIP or MVA.TIP_m4-1BBL, spleens were harvested 1 week later and the response to Pb9 measured by ELISpot. Responses to P15 were not above background levels. C & D) Balb/c mice were immunised either i.d. (C) or i.m. (D) with 108 iu of HAdV5.TIP-EGFP or HAdV5.TIP-EGFP. Spleens and serum were harvested 2 weeks later and the response to Pb9, EGFP and P15 measured by ELISpot and antibodies to GFP protein measured by ELISA. Data was analyzed with a 2-way analysis of variance with a post-hoc Bonferroni test; p values denote the level of statistical significance. For all graphs, the bar represents the median per group with each individual animal displayed as a single point.