Figure 5. Coadministration of vectors expressing 4-1BBL.

A) C57BL/6J mice were vaccinated i.m. with 2.8×10⁶ iu of HAdV5.MSP either alone or mixed with 10⁷iu of HAdV5.TIP-EGFP or HAdV5.TIP-EGFP 4-1BBL. Spleens were harvested 2 weeks later and the response to TIP antigen SIV, a pool of CD8⁺ T cell epitopes from MSP or a pool of CD4⁺ T cell epitopes from MSP measured by IFN-γ ELISpot. B) In separate experiments, Balb/c mice were vaccinated i.m. with 10⁶ iu of ChAd63.ME-TRAP mixed together with different ratios of ChAd63-m4-1BBL (left) or with 10⁷iu of ChAd63.m4-1BBL, ChAd63.h4-1BBL or ChAd63.AMA-1 (right). Spleens were harvested 2 weeks later and the response to Pb9 measured by IFN-γ ELISpot. C) Balb/c mice were immunised i.m. with 10⁶iu ChAd63.ME-TRAP, ChAd63.AMA-1 or ChAd63.CS with or without 10⁷iu ChAd63.m4-1BBL. Spleens were harvested 2 weeks later and antigen specific IFN-γ responses to the relevant antigens measured by ELISpot. For all experiments bars represent the median per group with each individual animal displayed as a single point. Each experiment was analysed with a one-way analysis of variance a post-hoc Bonferroni test (A&B) or an unpaired t-test (C); the p values denote the level of statistical significance.