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. 2014 Aug 20;9(8):e105595. doi: 10.1371/journal.pone.0105595

Figure 5. Igf-1r hemizygosity has no effect on AKT and S6K activation or autophagosome numbers in HD mice.

Figure 5

A. Igf-1r deficiency leads to a significant decrease in IGF-1R protein levels (p = 0.0057) but does not significantly affect AKT phosphorylation at Ser473 or Thr308, nor the phosphorylation of S6K at Threonine 389 in half-brain homogenates of 12 weeks old female mice of the indicated genotypes. Representative images are presented. For densitometry analysis, four different samples for each genotype were used (n = 4 per group). Further analysis is presented in Figure S3. B. Igf-1r hemizygosity does not affect autophagosome numbers. Brain homogenates from 12 week-old females of the indicated genotypes were subjected to western blot analysis to assess LC3 levels. The graph reports the quantitative analysis of LC3-II levels relative to actin from four different brain samples for each genotype. The p values for the densitometric analyses were determined by using Student’s t-test (n = 4). Data are expressed as mean ± s.e.m. *P<0.05; **P<0.01; ***P<0.001. ns = non-significant.