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. 2014 Aug 20;9(8):e105582. doi: 10.1371/journal.pone.0105582

Table 1. Descriptions of target and reference genes.

Gene Abbrev. Speciesof Origin Top Matches in GenBank,Species of Origin PutativeFunction PrimerSequence (5′-3′)
(Accession No.) (Accession No. for Nucleotideor Peptide Sequence)
Putative xenobiotic metabolism related genes
CytochromeP450 15A1 CYP15A1 Coptotermes formosanus CYP151A, Reticulitermes flavipes(ACN93795) Epoxidase, ATTTGTCACTCGCCTTGGTC
FK835449 CYP151A, Diploptera punctata(AAS13464) juvenile hormonesynthesis AAAGTTGCCATACGTGGAGG
CytochromeP450 CYP450 Coptotermes formosanus CYP314a1, Drosophila melanogaster (Q9VUF8.3) Ecdysone 20-monooxygenase ACTGGTTTGTAGTCGGTGCC
FK833823 CYP12A2, Musca domestica(O18635.1) Monooxygenase,oxidoreductase TTAGCACCGAGAGACGTTCA
Glutathion-S-Transferase GST Hodotermopsis sjoestedti Glutathione S transferase,Blattella germanica Metabolism oflipophilic toxins, GTTTCAAGCCTCGCTTTTGAC
DC239424 (FJ855500.1) excretion ofxenobiotics CGTCACTGTGAGACTGCCAT
Multi antimicrobial extrusion MatE Coptotermes formosanus MatE, Trichonomonas vaginalis(XP_001314814.1) Sodiumantiporter,cationic efflux TTGACACAACACCTTTCGGA
protein FK833694 transport pump,excretion oforganic cations GCCGTGTTTGAGGATGAAAT
Putative Immune related genes
Chitin bindingprotein CBP Coptotermes formosanus chitin-binding protein,Holotrichia oblita(AFD28282.1) Peritrophin A-typechitin-bindingprotein CGGGAGGACTGCTAGAACTG
FK832766 TGAGGGAACTCCCTGTTCAC
Downsyndrome DSCAM Coptotermes formosanus Downsyndrome celladhesion molecule immunglobulin-like fibronectin, CCGTACCATTGACGGAGAGT
cell adhesionmolecule FK835436 Harpegnathos saltator(EFN81146.1) cell recognitionand adhesion, CACTTGTGTTGCCCGTAATG
axon guidancereceptor in insects
Ficolin-2precursor FICO-2 Coptotermes formosanus Ficolin-2 precursor, Pediculus humanus corporis,(XP_002430827.1) Antigenrecognition,carbohydrate binding, CTCCGTCGGACTCTCATCTC
FK836944 scabrous protein-like, Tribolium castaneum, (XP_972571.1) Enhancesphagocytosis andopsonization GCGCTATAACCTGGGTCTCA
Lectin-relatedhemolymph LECT-like Coptotermes formosanus Hemolymph lipopolysaccharide-binding protein, Periplaneta americana (P26305.1) E. coliLipopolysaccharidebinding, CTTCCCAGTGTCGAAAGCTC
Lipospolysaccharide bindingprotein FK834461 Lectin-related regenectin,P. americana (BAA82265.1) Tissueregeneration TGGGTACCCCATAGGAGATG
C-type lectin CLECT Coptotermes formosanus conserved hypothetical C-type lectin, P. humanus corporis (XP_002424769.1) carbohydrate-binding, pathogenrecognition AGGGTGAAAGAGGAGCAACA
FK835521 innate immunity TAAAAATCCCAACAACCCCA
Gram-negativebinding protein 2 GNBP-2 Drepanotermes rubriceps gram negative bacteria binding protein 2 from different termite species insect pathogenrecognition protein CTTCCCAGTGTCGAAAGCTC
DQ058934 (e.g., AAZ0893.1-AAZ08504.1, AEK64801.1, ADJ19004.1) beta(1,3)-glucanase effector TGGGTACCCCATAGGAGATG
Reference genes
Cytoplasmicheat shockprotein 70 HSP Coptotermes formosanus ATP andnucleotide binding AGCCTTGGCCACAACAGTGCAA
FK835495 GGAGCAGGAGCAGGACCGACT
NADHDehydrogenasesubunit 4 NADH Coptotermes formosanus Mitochondrialelectron, sodiumion and ACGAAGCAACCCATAACCACCAAGC
FK833785 proton transport,ubiquinone activity GGGCTCATGTTGAGGCTCCTGTT
Elongationfactor1-alpha EFA Coptotermes formosanus GTP binding, GGCGGTCACATTTCTCCTTA
FK834645 regulation oftranslationalelongation CTGAACCACCCTGGTCAGAT