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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Mucosal Immunol. 2014 Mar 12;7(5):1255–1265. doi: 10.1038/mi.2014.15

Figure 6. SAMP-F Tregs are resistant to E2-mediated immunosuppression.

Figure 6

(A, B) CD4+CD25− Tconv cells were isolated from 10-wk-old SAMP mice by FACS sorting. Sorted cells were labeled with eFluor 670 cell proliferation dye and cultured in vitro with αCD3/αCD28 in the presence or absence of E2, PPT, or DPN (10nM) for 3 days. Proliferation was assessed by e670 dye dilution. (A) Representative histograms show the proliferation of viable, CD4-gated cells in the absence (white peaks) and presence (gray peaks) of E2. Cumulative statistics show the percentage of dividing cells in the presence of E2 (*p<0.002, n=3/group). (B) Percent proliferation of E2-, PPT-, or DPN-treated cells was calculated relative to percent proliferation of untreated cells using dye dilution and gating strategy shown in (A). Results are expressed as mean ± SEM (*p<0.001, n=3/group).