Table 2. Regional expression of Kv2.1 juxtaposed clustering with RyR.
Multiple label immunofluorescence was performed on serial coronal brain sections for Kv2.1 and RyR. Sections that included the striatum were scored for levels of Kv2.1 per cell, with the highest levels being scored as four:plus signs (++++), equal to the intensities found in striatal MSNs. Regions were also noted for having a majority of the cells exhibiting somatic clustering of RyR. Levels of RyR were not indicated due to heterogeneity within structures.
| Brain Region |
Kv2.1
expression |
Somatic
Clustered RYR |
|---|---|---|
| Cortex | +++++ | |
| Hippocampus | ||
| Pyramidal neurons | ++++ | Yes |
| Dentate Gyrus | ++++ | Yes |
| Subiculum | ++++ | |
| Habenular complex | ||
| Medial habenular nucleus (MHb) | +++ | |
| Lateral habenular nucleus (LHb) | ++ | |
| Amygdala | ||
| Lateral amygdaloid nucleus | ||
| Dorsolateral part (LaDL) | ++++ | |
| Ventrolateral part (LaVL) | ++++ | |
| Central amygdaloid nucleus | ||
| capsular part (CeC) | ++ | Yes |
| lateral division (CeL) | ++ | Yes |
| Striatum | ||
| Caudate putamen (Cpu) | ++++ | Yes |
| Globus pallidus (GP) | + | |
| Thalamus: Anterior nuclear group | ||
| Anteroventral nucleus (AV) | +++ | Yes |
| Anteromedial nucleus (AM) | ++ | Yes |
| Thalamus: Mediodorsal nucleus (MD) | ||
| Intermediate level | ||
| Medial part (MDM) | ++ | Yes |
| Central part (MDC) | ++ | Yes |
| Lateral part (MDL) | +++ | Yes |
| Thalamus: Ventral nuclear complex | ||
| Ventromedial nucleus (VM) | +++ | Yes |
| Ventrolateral nucleus (VL) | ++ | Yes |
| Ventral posterolateral nucleus (VPL) | ++ | Yes |
| Ventral posteromedial nucleus (VPM) | ++ | Yes |
| Thalamus: Lateral nuclear group | ||
| Laterodorsal nucleus (LD) | ||
| Dorsomedial part (LDDM) | ++++ | |
| Ventrolateral part (LDVL) | +++ | |
| Lateroposterior nucleus (LP) | ||
| Laterorostral part (LPLR) | +++ | |
| Mediorostral part (LPMR) | +++ | |
| Thalamus: Posterior nucleus (Po) | +++ | Yes |
| Thalamus: Intralaminar nuclear group | ||
| Central medial nucleus (CM) | +++ | Yes |
| Centrolateral nucleus (CL) | ++ | |
| Parafascicular nucleus (PF) | +++ | |
| Thalamus: Midline nuclear group | ||
| Paraventricular nucleus (PV) | + | |
| Ineranteromedial nucleus (IAM) | ++ | |
| Intermediodorsal nucleus (IMD) | ++ | Yes |
| Thalamus: Reticular nucleus (Rt) | ++ | |
| Thalamus: Geniculate Nucleus | ||
| Pregeniculate nucleus | ||
| Magnocellular part (PGMC) | ++ | |
| Parvicellular part (PGPC) | +++ | |
| Lateral geniculate nucleus | ||
| Dorsal part (DLG) | +++ | Yes |
| Anterior pretectal nucleus | ||
| Dorsal part (APTD) | +++ | |
| Sub-thalamus: Zona incerta (ZI) | ||
| Dorsal part (ZID) | ++ | |
| Ventral (ZIV) | ++ | |
| Subthalamic nucleus (STh) | +++ | Yes |
| Parasubthalamic nucleus | ++ |