Table 3. Biophysical properties of Kv2.1 in response to RyR expression and stimulation.
The midpoints of activation and inactivation (V1/2) and slope factors (k) were obtained from a single Boltzmann fit. Values are mean ± SE and n represents the number of experiments. Values in bold font are statistically significant (p < 0.05) compared with the corresponding Kv2.1 properties before treatment with 1 NM ionomycin (IM) or 1 NM ryanodine (Ry).
| Activation | Inactivation | |||||
|---|---|---|---|---|---|---|
| V1/2 | k | n | V1/2 | k | n | |
| Kv2.1 | 1.06 ± 0.55 | 9.35 ± 0.47 | 16 | −27.17 ± 1.43 | 6.05 ± 0.30 | 7 |
| + IM | −9.03 ± 2.00 | 7.95 ± 1.00 | 6 | −25.58 ± 1.22 | 5.58 ± 0.67 | 5 |
| + Ry | −2.36 ± 4.39 | 11.27 ± 0.81 | 5 | −28.34 ± 1.65 | 5.27 ± 0.24 | 5 |
| Kv2.1 + RyR1 | 0.00 ± 1.75 | 10.10 ± 0.68 | 15 | −27.98 ± 2.94 | 5.80 ± 0.25 | 6 |
| + Ry | −10.84 ± 3.04 | 9.94 ± 0.71 | 6 | −30.47 ± 3.71 | 5.40 ± 0.19 | 6 |
| Kv2.1 + RyR2 | −3.09 ± 0.94 | 8.55 ± 0.42 | 21 | −30.31 ± 2.64 | 5.37 ± 0.14 | 12 |
| + Ry | −15.25 ± 2.43 | 9.18 ± 1.09 | 6 | −32.25 ± 3.07 | 5.31 ± 0.16 | 6 |
| Kv2.1 + RyR3 | −0.89 ± 1.76 | 8.85 ± 0.68 | 20 | −24.96 ± 2.56 | 5.67 ± 0.43 | 5 |
| + Ry | −14.43 ± 2.82 | 8.85 ± 0.68 | 6 | −33.72 ± 2.79 | 5.63 ± 0.28 | 4 |