Figure 5. Ablation of Wls in liver epithelial cells has no effect on hepatic zonation or initiation of LR.
A. IHC for GS, Cyp1a2 and Cyp2e1 in Con and Wls-LKO shows normal pericentral localization in both groups. (100×)
B. PCNA IHC shows comparable numbers of PCNA-positive hepatocytes in Con and Wls-LKO livers at 40 hours after PH (200×).
C. Quantification of PCNA staining confirms similar numbers of PCNA-positive hepatocytes in Con and Wls-LKO at 40 hours after PH.
D. Representative WB shows comparable Cyclin-D1 expression at 40 hours after PH in both Con and Wls-LKO livers.