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. 2014 Apr 25;60(4):280–287. doi: 10.1262/jrd.2013-124

Table 1. Primer pairs used for the detection of mRNA.

Genes Primer sequence (5′→ 3′) Size (bp) Annealing
temp. (C)
Genbank Accession No.
LPS receptor
TLR4 For: CTT GCG TAC AGG TTG TTC CTA A 153 56 NM174198
Rev: CTG GGA AGC TGG AGA AGT TAT G
CD14 For: GGG TAC TCT CTG CTC AAG GAA C 199 56 NM174008
Rev: CTT GGG CAA TGT TCA GCA C
MD2 For: GGG AAG CCG TGG AAT ACT CTA T 204 54 DQ319076
Rev: CCC CTG AAG GAG AAT TGT ATT G
Progesterone production
StAR For: GTG GAT TTT GCC AAT CAC CT 203 58 NM174189
Rev: TTA TTG AAA ACG TGC CAC CA
Androstenedione production
CYP17 For: TGG ATC GTG GCC TAC CTC CT 215 58 M12547
Rev: AGG TCG CCA ATG CTG GAG TC
Gonadotropin receptor
LHr For: AGG AAA ATG CAC GCC TGG AG 202 58 U20504
Rev: GTG GCA TCC AGG AGG TTG GT
Internal standard
GAPDH For: CTC TCA AGG GCA TTC TAG GC 120 58 U85042
Rev: TGA GAA AGT CGT TGA GG