Table 1. Primer pairs used for the detection of mRNA.
Genes | Primer sequence (5′→ 3′) | Size (bp) | Annealing temp. (C) |
Genbank Accession No. | |
LPS receptor | |||||
TLR4 | For: CTT GCG TAC AGG TTG TTC CTA A | 153 | 56 | NM174198 | |
Rev: CTG GGA AGC TGG AGA AGT TAT G | |||||
CD14 | For: GGG TAC TCT CTG CTC AAG GAA C | 199 | 56 | NM174008 | |
Rev: CTT GGG CAA TGT TCA GCA C | |||||
MD2 | For: GGG AAG CCG TGG AAT ACT CTA T | 204 | 54 | DQ319076 | |
Rev: CCC CTG AAG GAG AAT TGT ATT G | |||||
Progesterone production | |||||
StAR | For: GTG GAT TTT GCC AAT CAC CT | 203 | 58 | NM174189 | |
Rev: TTA TTG AAA ACG TGC CAC CA | |||||
Androstenedione production | |||||
CYP17 | For: TGG ATC GTG GCC TAC CTC CT | 215 | 58 | M12547 | |
Rev: AGG TCG CCA ATG CTG GAG TC | |||||
Gonadotropin receptor | |||||
LHr | For: AGG AAA ATG CAC GCC TGG AG | 202 | 58 | U20504 | |
Rev: GTG GCA TCC AGG AGG TTG GT | |||||
Internal standard | |||||
GAPDH | For: CTC TCA AGG GCA TTC TAG GC | 120 | 58 | U85042 | |
Rev: TGA GAA AGT CGT TGA GG |