Fig. 2.

Transient transfection assay of mouse Prrx1 and Prrx2 promoters. Reporter vectors of Prrx1 (A) and Prrx2 (B) were transfected into CHO, NIH3T3 and TtT/GF cells. An aliquot of cultured medium was used for the SEAP assay. Reporter gene activities are indicated relative to that of the pcDNA3.1 vector. Representative data (mean ± SD) are shown from means of quadruplicate transfections from two independent experiments. Asterisks indicate statistical significance by one-way ANOVAs with Dunnett’s test (*P<0.05). (C) RT-PCR was performed for Prrx1, Prrx2, Klf6 and Tbp (TATA-box binding protein).