Table 1. List of primers used for construction of the 5’ upstream region of Prrx1 and Prrx2.

Prrx1
Forward primer	–2297	5’-aatacgcgtTCTAGAACAATGGGGGAG-3’
	–1400	5’-aatacgcgtAGTGGCTGGAGCGAGGCG-3’
	–1140	5’-aatacgcgtTCTCATAGCTACAGGAGAAG-3’
	–450	5’-aatacgcgtTCTCCGCCAAAACAAAGCTG-3’
Reverse primer	+103	5’-acacactcgcgaTCCACTTAATAGGAGCCTGTA-3’
Prrx2
Forward primer	–5060	5’-aatacgcgtAGGAGGATTTGTGTGGCTTG-3’
	–3567	5’-aatacgcgtGGGCACCAGGAACATTTGTA-3’
	–1752	5’-aatacgcgtACACACCAGAAGAGGGCATC-3’
	–1091	5’-aatacgcgtAGGCTCTGGACCTCACTTTG-3’
	–372	5’-aatacgcgtCAAATTCGAGGCTAATCTGC-3’
Reverse primer	+21	5’-acacactcgcgaGTGCCGGATCTCAAGTCAGT-3’

The uppercase and lowercase letters indicate the sequence of the gene to be amplified and adaptor containing the recognition sequence for restriction enzymes (Mul I for the forward primer and Nru I for the reverse primer, respectively).