Table 2. List of primer sets to amplify full-length cDNA.
Factor | Species | Accession Number | Forward primer | Reverse primer |
Prop1 | Rat | NM_153627.1 | CGGAATTCGCCATGCGGTCACTACTCCAGCCA | CCGGCTCGAGTTACTCTTGCTTCCGTTGCTTAGCC |
Prrx1 | Rat | NM_153821.1 | GAGAATTCGCGATGTTTTATTTCCACTG | GGAGCTCGAGTTCATCCTTGTGTCATATC |
Prrx2 | Rat | NM_001105739.1 | CCGGGAATTCGGCATGGACAGCGCGGCCGCCGCCTT | CCGGCTCGAGTGTCAGTTCACTGTGGGCACC |
SOX2 | Rat | NM_001109181.1 | GGAGAATTCGCCATGTATAACATGATGGAGACG | GGACTCGAGTCACATGTGCGACAGGGG |
Lhx2 | Mouse | NM_010710.3 | AGAGAGAATTCGCGATGCTGTTCCACAGTC | ACACACTCGAGTTGGGGGGTGGCGAGTC |
Lhx3 | Rat | XM_001059910 | GGAATTCGCCATGGAAGCTCGCGGGGAGCT | TCTCTCGAGTCAGAACTGAGCATGGTCTAC |
Isl1 | Mouse | NM_021459.4 | AGAGACAATTGGATATGGGAGACATGGGCGA | TGTGTCTCGAGCCTCATGCCTCAATAGGACTGG |
Otx2 | Mouse | NM_001286481.1 | AGAGAGAATTCAGCATGATGTCTTATCTAAAGCAACCGC | TGTGTCTCGAGACAGGTCTTCACAAAACCTGGAA |
Pax6 | Mouse | NM_001244198.1 | GACAATTGAGCATGCAGAACAGTCACAGC | ACGCTCGAGCTCTCTCCTTCTCTCTTTA |
Foxj1 | Mouse | NM_008240.3 | GACAATTGGACATGGCGGAGAGCTGGCTG | ACGCTCGAGACCTTTTACAAGAAGGCAC |
Hey1 | Mouse | NM_010423.2 | GAGAATTCACCATGAAGAGAGCTCACCCAG | ACGCTCGAGCATCAGTTCTTTAGAAAGC |
Hey2 | Mouse | NM_013904.1 | GAGAATTCGCGATGAAGCGCCCTTGTGAGG | ACGCTCGAGATTCAAGAATAAGTTAAAAG |
Id3 | Mouse | NM_008321.2 | AGAGAGAATTCAACATGAAGGCGCTGAGCCC | TGTGTCTCGAGTTCGGGAGGTGCCAGGACGAC |
Klf6 | Mouse | NM_011803.2 | GAGAATTCAGCATGAAACTTTCACCTGCG | ACGCTCGAGCCTACAGGATTCGTCCCTC |
Nfib | Mouse | NM_008687.6 | GAGAATTCGTCATGATGTATTCTCCCATCTGTCTCACTCAGGATGAG | GCGTCGACCAAGCTAGCCCAGGTACC |
Nkx3-1 | Mouse | NM_010921.3 | AGAGAGAATTCGGGATGCTTAGGGTAGCGG | TGTGTCTCGAGTTGCTACCAGAAAGATGGATGC |
Runx1 | Mouse | NM_001111021.2 | GAGAATTCAAGAGTGCGAGTCTGCCTGTG | ACGCTCGAGTCCCTCGATGGCGATGGCGC |
Sp1* | Human | BC062539.1 | GAGAATTCACCATGAGCGACCAAGATCACT | TCCTCGAGCTACCTTGCATCCCGGG |
Tal1 | Mouse | NM_011527.3 | AGAGAGAATTCACGATGACGGAGCGGCCGCCGAGC | TGTGTCTCGAGTCACATAAGTCCATTGACCTGCTT |
Tcf7l2 | Mouse | NM_001142918.1 | AGAGAGAATTCAAAATGCCGCAGCTGAACGGC | TGTGTCTCGAGCCTAGCAGATGCGGTG |
Tead2 | Mouse | NM_001285498.1 | GAGAATTCAAGATGGGGGATCCCCGGACTG | ACGCTCGAGCTCCATCAGTCCCTGACCAGG |
Zfp36 | Mouse | NM_011756.4 | GAGAATTCACCATGGATCTCTCTGCCATC | ACGCTCGAGGCACTTGTCACTCAGAGAC |
Zfp36l1 | Mouse | NM_007564.5 | GAGAATTCAGCATGATCCGCGGCGCCCCGGCAC | ACGCTCGAAGTCAGTGTGAGGGCTACTGGCGGAG |
* The human SP1 cDNA clone was kindly provided by Dr. R. Tijan of the University of California at Berkeley.