Table 3. Transcriptional activity of the 5’ upstream region of Prrx1 and Prrx2 in the presence of transcription factors.
|
Prrx1 |
Prrx2 |
|||
| –2297 | –450 | –5060 | –372 | |
| PROP1 | 1.29 ± 0.20 | 0.87 ± 0.15 | 0.23 ± 0.29** | 0.42 ± 0.23** |
| PRRX1 | 0.80 ± 0.20 | 0.53 ± 0.17** | 0.90 ± 0.17 | 0.51 ± 0.20** |
| PRRX2 | 1.75 ± 0.21** | 0.94 ± 0.26 | 4.11 ± 0.22** | 0.79 ± 0.10 |
| SOX2 | 3.31 ± 0.13** | 0.89 ± 0.18 | 0.39 ± 0.10 | 0.46 ± 0.20** |
| LHX2 | 1.74 ± 0.17* | 1.31 ± 0.17 | 1.08 ± 0.22 | 0.93 ± 0.11 |
| LHX3 | 1.41 ± 0.21 | 1.54 ± 0.26* | 0.62 ± 0.18** | 1.44 ± 0.24 |
| ISL1 | 3.94 ± 0.16** | 1.67 ± 0.02** | 1.01 ± 0.26 | 1.12 ± 0.11 |
| OTX2 | 1.73 ± 0.11** | 1.41 ± 0.16* | 0.49 ± 0.04** | 0.60 ± 0.29* |
| PAX6 | 2.21 ± 0.17** | 1.39 ± 0.14 | 2.72 ± 0.12** | 1.40 ± 0.02** |
| FOXJ1 | 0.27 ± 0.11** | 0.61 ± 0.06** | 0.16 ± 0.10** | 0.53 ± 0.05** |
| HEY1 | 0.52 ± 0.16** | 0.59 ± 0.10** | 0.06 ± 0.16** | 0.39 ± 0.08** |
| HEY2 | 0.52 ± 0.08** | 0.94 ± 0.11 | 0.16 ± 0.27** | 0.43 ± 0.06** |
| ID3 | 1.09 ± 0.28 | 0.81 ± 0.25 | 0.43 ± 0.21** | 0.38 ± 0.11* |
| KLF6 | 0.75 ± 0.07** | 1.05 ± 0.13 | 18.23 ± 0.13** | 6.36 ± 0.21* |
| NFIB | 1.05 ± 0.16 | 0.55 ± 0.22** | 0.75 ± 0.23** | 1.07 ± 0.13 |
| NKX3-1 | 1.40 ± 0.22 | 0.83 ± 0.11 | 1.52 ± 0.20* | 0.80 ± 0.10 |
| RUNX1 | 0.87 ± 0.08 | 0.88 ± 0.21 | 0.80 ± 0.12 | 1.13 ± 0.20 |
| SP1 | 3.69 ± 0.20** | 2.21 ± 0.20** | 7.87 ± 0.16** | 4.83 ± 0.07** |
| TAL1 | 0.79 ± 0.17* | 0.80 ± 0.08* | 0.97 ± 0.20 | 0.89 ± 0.04 |
| TCF7L2 | 1.21 ± 0.11* | 0.83 ± 0.28 | 1.86 ± 0.06** | 0.88 ± 0.11 |
| TEAD2 | 0.58 ± 0.22** | 0.71 ± 0.14** | 0.31 ± 0.07** | 0.79 ± 0.24 |
| ZFP36 | 0.44 ± 0.18** | 0.39 ± 0.26** | 0.25 ± 0.14** | 0.69 ± 0.20** |
| ZFP36L1 | 0.63 ± 0.14** | 0.89 ± 0.24 | 0.40 ± 0.15** | 1.03 ± 0.04 |
| KLF6+SP1 | 1.04 ± 0.15 | 0.77 ± 0.07* | 26.27 ± 0.12** | 6.40 ± 0.14** |
Transcriptional activity was assayed in CHO cells by transfection of reporter vectors, Prrx1 (–2297/+103), Prrx1 (–405/+103), Prrx2 (–5060/+21) and Prrx2 (–372/+21), and an expression vector harboring cDNA encoding the transcription factors listed in the Table. Data are indicated as the relative activity against that without the expression vector, and representative data are shown as means ± SD of quadruplicate transfections in two independent experiments. Asterisks indicate statistical significance by Student’s t-test (*P<0.05; **P<0.01).