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. Author manuscript; available in PMC: 2014 Aug 21.
Published in final edited form as: Biochemistry. 2013 Apr 15;52(16):2821–2827. doi: 10.1021/bi400212m

Figure 1. Collagenase I-digestion releases a 170-kDa TGFBIp-containing complex from the SDS-insoluble fraction of porcine cornea.

Figure 1

Porcine cornea powder was washed extensively and digested with collagenase I for different periods of time at 37°C. The supernatant from the first wash (0 min), and supernatants after digestion for 10 min, 90 min, and 8 hours were analyzed by anti-TGFBIp immunoblotting following non-reducing SDS-PAGE. Collagenase auto digest (no cornea powder) was loaded in the final lane (Collagenase). Molecular weights are indicated on the right. The immunoblot shows that there is a time-dependent release of a major 170-kDa and a minor 210-kDa TGFBIp-containing molecule when corneal tissue is incubated with collagenase I.